Senior / Principal Scientist, Biochemistry

Posted 7/29/2026 · last confirmed live 8/26/2026

Senior / Principal Scientist Biochemistry   About Treeline Treeline Biosciences is an innovative biotechnology company, founded by an experienced team of biopharmaceutical scientists and executives, committed to developing new medicines against difficult to drug targets in cancer and other life-threatening diseases. We are pursuing a uniquely integrated approach that applies modern and emerging tools in genomics, lead discovery, medicinal chemistry, and computation to reduce the time and risks of drug discovery.   About the Role We are seeking an experienced and enthusiastic biochemist to join our growing team.  The successful candidate for the Senior / Principal Scientist, Biochemistry position will have a critical role in supporting Treeline’s drug discovery programs by collaborating closely with a multi-disciplinary team of biology, biophysics, structural biology, computational, and medicinal chemistry scientists to deliver high-quality results and drive small molecule drug discovery.  Specifically, you will propose, design, and implement new biochemical assays to screen and characterize protein-small molecule and protein-protein interactions.   Responsibilities Propose, design, and implement biochemical assays for compounds testing and mechanism-of-action studies. Lead from the bench to propose and develop new assays and drive hit identification, hit-to-lead, and lead optimization experiments in collaboration with a cross-functional project team. Design and conduct high-quality scientific experiments. Apply biochemical methods to enable mechanistic studies and determine the mode of inhibition for promising hits. Analyze, interpret, and communicate high-quality data to a cross-functional project team. Collaborate with chemistry, biology, lead discovery, and medicinal design teams to characterize and establish SAR, rapidly advancing the hit-to-lead process. Propose and evaluate new approaches and technologies. Provide scientific leadership, mentorship, and cross-functional partnership within a dynamic matrixed environment.   Required Qualifications PhD in biochemistry or a related discipline with 5+ years of industry experience, or a comparable combination of postdoctoral training and industry experience. Strong knowledge and understanding of steady-state enzyme kinetics and binding assays. Experience in small molecule drug discovery, specifically biochemical assay development and characterization of small molecule inhibitors and protein–ligand binding interactions. Experience in a wide array of biochemical assays, both enzymatic and binding, such as FP, TR-FRET, AlphaLISA, DSF. Experience with validating hits coming from DEL screening campaign Strong attention to detail, with excellent troubleshooting and problem-solving skills. Strong written and oral communication skills, excellent interpersonal skills, with demonstrated ability to work collaboratively on multidisciplinary teams.   Preferred Qualifications Knowledge of automation and experience with assay validation for HTS is beneficial. Experience using numerical simulations for experiment set up and data analysis Experience in working with challenging proteins. Working knowledge of adjacent disciplines (biophysics, structural biology, protein purification, biology, chemistry). General knowledge of signaling pathways, protein homeostasis, and regulation of gene expression beneficial. Track record of publications in high-impact scientific journals. This position is classified as exempt. The anticipated annual base salary range for candidates who will work in Watertown is $158,000 to $184,000. The final base salary offered to the successful candidate will be dependent upon several factors that may include but are not limited to the type and length of experience within the job, and other factors. Treeline Biosciences is a multi-state employer, and this salary range may not reflect positions that work in other cities or states.   Recruitment fraud statement Please be aware of recruitment fraud and related job scams, where scammers present themselves as recruiters but are seeking to steal money or personal information. Keep the following in mind to protect yourself: Treeline Biosciences will never ask for money from you as part of our recruitment process. Do not provide bank details or pay somebody for the promise of a job at Treeline. We do not conduct interviews through RingCentral, Skype or Telegram. Our job openings are first posted to treeline.bio/careers. If you locate a listing for a Treeline job on another site, please ensure it is also posted on our careers website. You can find more information about job scams at consumer.ftc.gov/articles/job-scams. To report job scams, head to ReportFraud.ftc.gov.     Notice to Search Firms/Third-Party Recruitment Agencies (Recruiters) The Human Resources team manages the recruitment and employment process for Treeline Biosciences. To protect the interests of all parties involved, Treeline Biosciences will only accept resumes from a recruiter if an executed search agreement directed to the particular position or positions is in place at the start of the recruitment effort. Unsolicited resumes sent to Treeline Biosciences from recruiters do not constitute any type of relationship between the recruiter and Treeline Biosciences and do not obligate Treeline Biosciences in any way to pay fees should we hire from those resumes. Recruiters are requested not to contact or present candidates directly to our hiring manager or employees.

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